Poste basé à Limoges

Employeur

The CRIBL unit (UMR CNRS7276 / INSERM1262) is located in Limoges, France, in a recent and spacious building. It is made up of about 60 people including researchers, students, engineers/technicians and clinicians. The theme of the unit is focused on the B lymphocyte in a normal or pathogenic context (lymphoproliferations).

The BioPIC team in which the post-doc will be recruited is made up of around 20 people and is more particularly interested in plasma cells and immunoglobulin deposition diseases. It is backed by the French National reference center for AL amyloidosis. The work will be done in collaboration with several academic and private partners (France, USA, Germany, Italy…)

Missions : AL Amyloidosis is an incurable disease caused by the deposition in the organs of insoluble fibrils composed of a monoclonal immunoglobulin (Ig) light chain (LC) produced by proliferating plasma cells. Whether formation of amyloid fibrils in general and AL fibrils in particular have been extensively studied in vitro, there is still a lack of reliable experimental in vivo models. We have recently uncovered the conditions to trigger AL amyloidosis in mice. This model will help understanding the formation of amyloid fibrils for the first time in vivo. It is also an exquisite tool to design and evaluate new therapeutic strategies in preclinical studies (ongoing collaborations with private and academic partners).
Activities :
The applicant will be in charge of the biochemical and immunological characterization of the amyloid fibrils (in vitro and in vivo) and amyloid deposits in tissues (Histopathology, Mass spectrometry, RNAseq, Spatial transcriptomic). He/She will use different systems to produce new Ig LCs and will improve the current mouse model with additional transgenic approaches (Crispr-Cas9 modifications in ES cells).
Mobilité géographique : Internationale

Télétravail : Occasionnel

Profil

We are seeking a PhD with strong expertise in immunology, antibodies and B cells. Skills in biochemistry of proteins, structural biology, production of recombinant proteins (prokaryotic and eukaryotic) and/or transgenesis (transfection, ES cells) and/or animal experimentation will be appreciated. General knowledges in physiology (heart, kidney) are also expected.
Objectifs
  • To improve the current AL amyloidosis mouse model and to create new ones (transgenesis)
  • To characterize the AL amyloidosis models (animal and cellular models)
  • If possible, to bring to skills and knowledge to the team about the biochemical and structural characterization of the immunoglobulin deposits (Biochimistry, Structural Biology, Mass spectrometry)
  • To participate to the avaluation of new therapeutic approaches for AL amyloidosis and other Ig deposition diseases.
Plus d’informations